Synechocystis sp. PCC6803 : sll1450

ORF IDsll1450
Gene namenrtA
DefinitionABC-type nitrate/nitrite transport system substrate-binding protein
Length446 aa
1341 bp   +upstreambp   +downstreambp
CyanoBasesll1450 nrtA nitrate transport protein NrtA
UniProtP73452 Nitrate transport protein nrtA.
KOK02051 ABC.SN.S; NitT/TauT family transport system substrate-binding protein
Pfam / Prositepf:TAT_signal

CommentFUNCTIONs - PHYSIOLOGICAL-ROLE: Active transport of nitrate s - PROTEIN-FUNCTION: nitrate- and nitrite-binding lipoprotein `DED: ssimilarity with Synechococcus sp. PCC 7942 `REF: Aichi et al. 2001sPROTEIN-FAMILY: Super-family of ABC transporters sLOCALIZATION/STRUCTURE: crystal structure determined `REF: Koropatkin et sal. 2006sREGULATIONs - OPERON-STRUCTURE: nrtABCD-narB (nrtABCD-?ssl2814?-narB) `EXP: Northern shybridization nrtC-specific probe indicated the presence of a large stranscription unit / narB-specific probe yielded essentially the same shybridization profile / the size of the largest signal was close to the scalculated size of the nrt-ABCD-narB gene cluster. `REF: Aichi et al. 2001s - REGULATOR: NtcA binding site, 20 bp upstream from the -10 promoter selement of nrtA `EXP `REF: Aichi et al. 2001s - PROMOTER: promoter contained an inverted repeat carrying a LysR motif s(TN11A), centered at position -24 with respect to the NtcA-binding motif ssupposed to constitute the binding site for NtcB `REF: Aichi et al. 2001s - EXPRESSION: The expression of nrtABCD-narB is repressed by ammonium and sinduced by inhibition of ammonium assimilation, suggesting involvement of sNtcA. / Under inducing conditions, expression of the transcription unit is senhanced by nitrite, suggesting regulation by NtcB. `REF: Aichi et al. 2001sMUTANTS: -sOTHER COMMENTS: synergistic regulation of bicarbonate and nitrate transports`HYP `REF: Koropatkin et al. 2006
ABC transporters
1. Aichi M, Takatani N, Omata T (2001) Role of NtcB in activation of nitrate assimilation genes in the cyanobacterium Synechocystis sp. strain PCC 6803. J Bacteriol 183:5840-7. [PMID:11566981]
2. Kobayashi M, Takatani N, Tanigawa M, Omata T (2005) Posttranslational Regulation of Nitrate Assimilation in the Cyanobacterium Synechocystis sp. Strain PCC 6803. J Bacteriol 187:498-506. [PMID:15629921]
3. Koropatkin NM, Pakrasi HB, Smith TJ (2006) Atomic structure of a nitrate-binding protein crucial for photosynthetic productivity. Proc Natl Acad Sci U S A 103:9820-5. [PMID:16777960]
PubMed search
Homology BLAST     FASTA
3D Structure PDB     GTOP

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